Figure 1. Cell viability within arginine-depleted cell populations.

L1210 cells were cultured in arginine-free media with or without arginine supplementation for 6 days. Arginine was reintroduced into arginine-free cultures on day 6 as indicated by the arrows. Cell viability was determined by (A) Trypan blue exclusion, or (B) ATP quantification. For clarity, only data leading up to - and including – the maximal response is shown. Graphs show a representative experiment from three independent experiments with similar results. Error bars represent SD.