Figure 2. Sensitivity of canine lymphoid cells and osteosarcomas to prolonged arginine-deprivation.

Canine lymphoid cells and osteosarcomas (A–L, described in Table 1) were cultured in Arginine-free media for 6 days with the amino acids indicated below. Arginine was reintroduced into cultures on day 6 as indicated by the arrows. Cell viability was determined by ATP quantification. Blue squares = +1 mM Arginine, Red diamonds = Arginine-free, Green triangles = +1 mM Citruline, Purple crosses = +1 mM Ornithine. Graphs show a representative experiment from three independent experiments with similar results. Error bars represent SD.