TABLE 2.
m/z | Proposed structurea | Peak area in OD 4 peptidoglycan | Peak area in OD 2 peptidoglycan | Ratiob |
---|---|---|---|---|
969.5 | GlcNAc-MurNAc-AQKAA | 471947 | 23702 | 20 |
| ||||
1040.5 | GlcNAc-MurNAc-AQK(A)AA | 245256 | trace amount | |
898.5 | GlcNAc-MurNAc-AQKA | 74923 | trace amount |
All the Lys (K) in the muropeptides in this study are isotopically enriched as [ε-15N]Lys;
Ratio of the peak area in the OD 4 and OD 2 peptidoglycan. Both peptidoglycan samples were adjusted to the same concentration by A600, and were prepared and injected the same way.