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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Pharm Res. 2012 Oct 23;30(2):489–501. doi: 10.1007/s11095-012-0895-1

Figure 3. Quantitative PCR Analysis of CYP2B6 mRNA induction.

Figure 3

HPHs isolated from 4 donors (HL-#32, -#33, -#35, and -#37) were cultured in Williams’ E medium and treated with vehicle control (0.1% DMSO), CITCO (1 μM), and test compounds at 10 μM each (A) or multiple concentrations (B and C) for 24 hr. Real-time PCR was employed to detect CYP2B6 induction as described in Materials and Methods. CYP2B6 inducibility of each compound was normalized to that of CITCO. Correlation between hCAR1+A activation and CYP2B6 induction was depicted in D. Data are expressed as mean ± SD (n=3). Dashed line indicates 40% of CITCO-mediated CYP2B6 induction.