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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Pharm Res. 2012 Oct 23;30(2):489–501. doi: 10.1007/s11095-012-0895-1

Figure 4. Induction of CYP2B6 protein in human primary hepatocytes.

Figure 4

Cultured HPHs were treated with vehicle control (0.1% DMSO), CITCO (1 μM), and test compounds at 10 μM each (A) or multiple concentrations (B) for 72 hr. After harvesting, cell homogenates (20 μg/each) were subjected to CYP2B6 protein Immunoblotting analysis as described in Materials and Methods. Densitometry analysis was performed using FluorChem SP Imaging System (C). Correlation between hCAR1+A activation and induction of CYP2B6 protein was demonstrated in D.