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. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: Pharm Res. 2012 Oct 23;30(2):489–501. doi: 10.1007/s11095-012-0895-1

HPHs from donor (HL-#37, and -#38) were infected with Ad/EYFP-hCAR and then subjected to eight hours of treatment with vehicle control (0.1% DMSO), the positive control, PB (1 mM), or each test compound at three indicated concentrations. Cellular localization of Ad/EYFP-hCAR was analyzed under confocal microscopy as outlined in Materials and Methods. Representative cellular distribution and chemical-mediated translocation were demonstrated (A). After treatment, one-hundred Ad/EYFP-hCAR expressing hepatocytes from donors (HL-#37, and -#38) were categorized into one of three groups: cytoplasmic, nuclear, or mixed (cytoplasmic + nuclear) (B).

HPHs from donor (HL-#37, and -#38) were infected with Ad/EYFP-hCAR and then subjected to eight hours of treatment with vehicle control (0.1% DMSO), the positive control, PB (1 mM), or each test compound at three indicated concentrations. Cellular localization of Ad/EYFP-hCAR was analyzed under confocal microscopy as outlined in Materials and Methods. Representative cellular distribution and chemical-mediated translocation were demonstrated (A). After treatment, one-hundred Ad/EYFP-hCAR expressing hepatocytes from donors (HL-#37, and -#38) were categorized into one of three groups: cytoplasmic, nuclear, or mixed (cytoplasmic + nuclear) (B).