FIGURE 5.

ARHGAP21 depletion affects the expression of proteins EMT markers during EMT induced by HGF. A, Western blotting (IB) shows ZEB1 and Snail1 expression in DU145 cells non-treated or induced with HGF for 30 min, 2 h, and 4 h. B, shown is expression of Twist1 in DU145 cells non-treated or induced with HGF for 10 min, 30 min, 2 h, and 4 h and control and ARHGAP21 siRNA stimulated with 4 h HGF. C, Western blotting shows E-cadherin, vimentin, and slug expression in control and ARHGAP21 DU145 cells non-treated or induced with HGF for 4 h. The Western blotting confirm that ARHGAP21 inhibition results in a diminishing of cells ability to undergo EMT in response to HGF signaling. Anti-β-actin blotting was used as loading control in A, B, and C.