FIGURE 6.

Reductions in S1PR1 or ITGB4 expression inhibits c-Met/S1PR1/ITGB4 complex activation. HPAEC were transfected with nonspecific (ns) siRNA or siRNA specific for S1PR1 or ITGB4 prior to treatment with HGF (25 ng/ml, 5 min). Cells lysates were immunoprecipitated with an anti-c-Met antibody and then subjected to immunoblotting with antibodies specific for c-Met, ITGB4, or S1PR1. Phosphorylation corresponding to the same molecular weight was determined via repeat immunoblotting on the same membrane with specific antibodies as shown (representative blots shown, densitometry data expressed as fold change phosphorylation relative to control and normalized to total protein, n = 3/condition, *, p < 0.05 compared with untreated nsRNA controls).