FIGURE 4.

Immunoblotting analyses of a B. napus PSV fraction separated by one-dimensional SDS-PAGE reveals cruciferin complexes of ∼48 to 58 kDa under non-reducing conditions. PSV fractions were separated under reducing conditions (A; +DTT) and non-reducing conditions (B; −DTT). Gels were either stained Coomassie colloidal or blotted onto nitrocellulose membranes and incubated with antibodies directed against the α or the β chains of cruciferin as indicated. Molecular masses of standard proteins are shown to the right and to the left in kDa.