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Analyses of cruciferin subunits by LC-MS/MS allows assigning clusters of spots on two-dimensional IEF/SDS gels to the three distinct cruciferin families. Names of families and polypeptide chain attribution are given to the right and below the gel. Molecular masses and pIs are given to the left and above the gel, respectively. P1, family 1 containing cruciferin Cru 1; P2, family 2 containing cruciferin BnC1, BnC2, and Cru 2/3; P3, family 3 containing cruciferin Cru 4.
