TABLE 1.

Characteristics of B. napus cruciferins and their α and β polypeptide chains

	No AA αa	No AA βa	No AA α+βa	MM cal α+βb	MM cal αb	MM app αc	MM cal βb	MM app βc	pI cal αb	pI app αc	pI cal βb	pI app βc
Cru 1	296	190	486	54.1	32.9	31.0–36.0	21.2	19.0–21.0	7.17	6.8–7.8	6.94	8.3–9.5
BnC1	254	190	477	51.4	30.6	27.0–36.0	20.8	19.0–21.0	6.78	6.8–7.8	6.16	5.9–7.0
BnC2	283	190	473	51.8	30.8	31.0–32.0	21.0	19.0–21.0	7.95	7.1–7.8	8.6	8.3–8.9
Cru 2/3	275	190	465	51.3	30.5	27.0–32.0	20.8	19.0–21.0	6.78	7.1	6.16	5.9–7.0
Cru 4	254	189	443	48.9	28.1	27.0–32.0	20.9	19.0–21.0	6.66	6.7–7.0	8.6	8.3–9.5

^a Number of amino acids, excluding the signal peptide, derived from the corresponding UniProt database entry.

^b Calculated isoelectric points and calculated molecular masses, excluding the signal peptide, using the ExPASy MM/pI tool.

^c Apparent isoelectric points and molecular masses upon analyses by two-dimensional IEF/SDS PAGE (Fig. 5).