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. 2012 Nov 27;288(4):2281–2289. doi: 10.1074/jbc.M112.393108

FIGURE 3.

FIGURE 3.

Effect of IDE silencing on neuroblastoma proliferation and viability. A, Western blotting analysis of IDE concentration in cell SHSY5Y lysates collected 48 h after stimulation either with an IDE-specific siRNA or a nontargeting siRNA pool. A representative immunoblot of six independent experiments is shown. Results presented are the means ± S.E. of six independent experiments. *, significantly different from control (p < 0.05, one way ANOVA, followed by Tukey's test, n = 18). B, after 48 h of transfection, SHSY5Y cells treated with the anti-IDE siRNA show a marked decrease of cell proliferation rate. Extensive cell death occurs 24 h later. C, proliferation rate is displayed for treated cells as the relative expression of untreated controls at 72 h after IDE-silencing. The results presented are the means ± S.E. of six independent experiments. *, significantly different from control (p < 0.05, one way ANOVA, followed by Tukey's test, n = 18). D, in the case of IDE-silenced cells at 48 h after silencing an increment of cleaved PARP-level is detected in the early stage of apoptosis.