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. 2012 Nov 27;288(4):2281–2289. doi: 10.1074/jbc.M112.393108

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FIGURE 5. — IDE effect on ubiquitin system. A, Western blotting of the overall poly-ubiquitinated proteins content in lysates from IDE-silenced SHSY5Y. IDE inhibition is accompanied by a significant reduction of the poly-ubiquitinated proteins compared with untreated or scrambled cells. A representative immunoblot of six independent experiments is shown. B, Western blotting analysis of co-immunoprecipitation sample in SHSY5Y. Immunoaffinity purification shows that IDE interacts with ubiquitin and 26S proteasome in SHSY5Y neuroblastoma cells. (Ctrl indicates the whole lysate.)