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Kinetic parameters of A. thaliana and C. roseus wild type and mutant enzymes
Values represent mean ± S.E. (n = 3).
| Organism | Enzyme | Vmax | Km | Substrate | Activity |
|---|---|---|---|---|---|
| nmol/min/mg protein | mm | ||||
| A. thaliana | PAAS (WT) | 112 ± 8 | 5.10 ± 0.70 | Phenylalanine | Aldehyde synthase |
| A. thaliana | PAAS (F338Y) | 341 ± 31 | 4.60 ± 0.55 | Phenylalanine | Decarboxylation |
| C. roseus | TDC (WT) | 2710 ± 110 | 0.12 ± 0.02 | Tryptophan | Decarboxylation |
| C. roseus | TDC (Y348F) | 160 ± 14 | 0.095 ± 0.02 | Tryptophan | Aldehyde Synthase |
