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Signaling through the MEK and PI3K pathways negatively regulates EGFR and NRas activity. Hec1A and DLD1 cells of the indicated genotypes were plated in 6-well dishes and at 70% confluence treated with the indicated concentration of the MEK inhibitor PD0325901 and the PI3K inhibitor GDC-0941. 18 h later, the cells were lysed, and 40 μg was subjected to SDS-PAGE and Western blotting using the antibodies shown.
