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. 2012 Dec 5;288(4):2441–2451. doi: 10.1074/jbc.M112.426700

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — A, Purified proteins used in this study. Lanes 1 and 5, molecular weight standards; lane 2, purified recombinant human cN II; lane 3, purified recombinant human cNIII-L; lane 4, purified recombinant Drosophila CG3362 protein; lane 6, partially purified recombinant human cNIII after SUMO cleavage. The band representing cNIII is marked with an asterisk. Molecular weight standards (M; kDa) are given in the margin. B, molecular weight determination of CG3362 by equilibrium sedimentation is shown. The experiment was carried out as described under “Experimental Procedures” with an initial protein concentration of 27.5 μm. The calculated and the experimentally determined molecular weights are given as an inset in the figure. r, radius. C, molecular weight determination of human cNIII-like by equilibrium sedimentation is shown. The experiment was carried out as in B, with an initial protein concentration of 29 μm.