FIGURE 2.

Voltage stimulus frequency-dependent NLC measurement. Examples of stimulus frequency-dependent NLC measurements on WT (A; n = 5) and 499ki OHCs (B; n = 5) are shown. NLC measurements were performed using five different f₁ frequencies with corresponding f₂ frequencies being twice as large as f₁. Equations 3 and 4 were used to determine the Q_max values of WT and 499ki OHCs, respectively. The obtained Q_max values were divided by those determined at 390.6 Hz, and resulting quotients were plotted as a function of the f₁ stimulus frequency in C. V_pk values were also plotted as a function of the f₁ stimulus frequency in D. Error bars represent S.D.