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. 2012 Dec 4;288(4):2452–2463. doi: 10.1074/jbc.M112.411579

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FIGURE 5. — Heteromer formation between WT- and 499-prestin. A, WT- and 499-prestin constructs used for the copurification assay. Wild-type prestin was tagged with an N-terminal FLAG epitope and C-terminal GFP, whereas the 499-prestin construct was tagged with a C-terminal myc epitope. B, copurification of 499-prestin with WT-prestin. The prestin constructs shown in A were singly or coexpressed in sf9 cells, purified using a FLAG affinity column, and analyzed by Western blot. The numbers indicate molecular masses in kDa.