FIGURE 2.

SST was specifically expressed in interneurons following activity elevation in vitro or in vivo. A, representative images of SST, GABA, and MAP2 immunostaining of DIV 12 primary hippocampal neurons, treatments as indicated. The scale bar is 50 μm. The number of neurons co-labeling with SST and GABA (SST⁺ GABA⁺), as a percentage of total SST immunopositive neurons (SST⁺) is quantitated to the right of each example. B, representative images of the CA1 region of GAD67-GFP mice, untreated or 24 h following KA injection, channels as labeled. The scale bar is 50 μm for the top row of each condition and 20 μm for the zoomed images (white boxes of top images) in the bottom row of each condition. The number of the neurons co-immunostaining with SST and GFP (SST⁺ GFP⁺), as a percentage of total SST immunopositive neurons (SST⁺), is shown to the right of each example. Ctrl, control; Bic., bicuculline.