FIGURE 3.
SST treatment reduced the density of dendritic spine, as well as that of pre- and postsynaptic markers of excitatory synapses, without affecting inhibitory synapses. A, representative images of the dendritic spines of control (Ctrl) neurons or those treated with SST. The scale bar is 5 μm. B, quantitation of dendritic spine density following SST treatment (control: 3.03 ± 0.19; SST: 2.22 ± 0.14, p < 0.01). C, SST treatment did not affect the size of dendritic spines: p > 0.05. D, E, H, and I, representative images of dendrites (labeled with MAP2 or GFP) immunostained with Bassoon and co-stained with vGluT1 (D), surface GluR2 (sGluR2, E), vGAT (H), or GABAARα1 (I). The dendritic segment within each white box is shown as a zoomed image below. The scale bar is 20 μm for full frame images and 5 μm for the zoomed images. F, SST treatment reduced the density of synaptic vGluT1 (control: 1 ± 0.05; SST: 0.80 ± 0.07, p < 0.05), without affecting the integrated intensity of vGluT1 puncta (control: 1 ± 0.05; SST: 0.96 ± 0.05, p > 0.05). G, SST treatment reduced the density of synaptic GluR2 (control: 1 ± 0.09; SST: 0.73 ± 0.07, p < 0.05), without affecting the integrated intensity of synaptic GluR2 puncta (control: 1 ± 0.04; SST: 1.02 ± 0.05, p > 0.05). J, SST treatment did not affect the density (control: 1 ± 0.11; SST: 1.04 ± 0.09, p > 0.05) or integrated intensity (control: 1 ± 0.07; SST: 0.94 ± 0.08, p > 0.05) of synaptic vGAT puncta. K, the density (control: 1 ± 0.06; SST: 0.98 ± 0.07, p > 0.05) or integrated intensity (control: 1 ± 0.05; SST: 1.05 ± 0.08, p > 0.05) of synaptic GABAARα1 was not affected by SST treatment. The results are shown as the means ± S.E. n, shown as white numbers in the bars, represents the number of neurons. *, p < 0.05; **, p < 0.01.