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. 2012 Dec 11;288(4):2501–2509. doi: 10.1074/jbc.M112.419051

FIGURE 5.

FIGURE 5.

The effects of SST on spines and mEPSCs were mediated by postsynaptic SSTR4. A, the SSTR4 RNAi (R4 RNAi) was efficient in reducing the level of SSTR4 in hippocampal neurons (R4 RNAi: 0.32 ± 0.08, p < 0.01). B, representative images of dendritic spines, conditions as indicated. The scale bar is 5 μm. C, SSTR4 RNAi blocked SST-induced reduction in dendritic spine density (control: 3.83 ± 0.21; SST: 2.98 ± 0.16, p < 0.05 versus control; R4 RNAi: 3.78 ± 0.22, p > 0.05 versus control; R4 RNAi + SST: 3.99 ± 0.24, p > 0.05 versus R4 RNAi). D, none of the treatments affected the size of dendritic spines: p > 0.05 for all conditions. E, representative mEPSC traces and average waveforms, conditions as indicated. F, SSTR4 RNAi blocked the effect of SST on reducing mEPSC frequency (control: 0.79 ± 0.16 Hz; SST: 0.36 ± 0.05 Hz, p < 0.05 versus control; R4 RNAi: 0.78 ± 0.15 Hz, p > 0.05 versus control; R4 RNAi + SST: 0.79 ± 0.15 Hz, p > 0.05 versus R4 RNAi). G, cumulative distribution of interevent intervals (SST versus control: p < 0.001; R4 RNAi versus control: p > 0.05; R4 RNAi + SST versus R4 RNAi: p > 0.05). H, SSTR4 RNAi did not affect mEPSC amplitude in control or SST-treated conditions. p > 0.05 for all conditions. The results are shown as the means ± S.E. n, shown as white numbers in the bars, represents the number of neurons. *, p < 0.05; **, p < 0.01. Ctrl, control.

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