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HD are required for viral infection. A, VP1 immunoblot of purified SV40 wild type and mutant viral particles to normalize infection levels used in B. Amino acids mutated in VP2/3/4 are indicated along with their location in the corresponding HD. B, percentage of infected cells was determined by flow cytometry 24 h postinfection and normalized to wild type SV40 using VP1 levels. Error bars represent the standard deviation of 30,000 cells measured in three independent experiments.
