FIGURE 1.

siRNA-mediated knockdown of GSAP in N2a cells. a, total RNA was extracted from different cell lines or rat brain tissue and the mRNA levels of GSAP and the housekeeping gene GAPDH were determined by quantitative RT-PCR. Data are mean ± S.D. GSAP expression is expressed as a % of the housekeeping gene GAPDH. b and c, N2a cells were transfected with GSAP siRNA or a scramble siRNA control (Ctrl). 48 h post-transfection, the effects on GSAP mRNA expression (b) and Aβ generation (c) were determined. Data are mean ± S.E. Statistical analysis was conducted using an unpaired t test or a one-way ANOVA with Dunnett's multiple comparison test (***, p < 0.0001).