FIGURE 3.

stau-1 is expressed throughout development. A, domain structure of STAU-1 protein. Brackets indicate regions deleted in stau-1 mutant alleles; stau-1(tm2266) deletes double-stranded RNA-binding domain 2 (referred to as stau-1(ΔdsRBD2)), and stau-1(q798) deletes double-stranded RNA-binding domain 4 (referred to as stau-1(ΔdsRBD4)). A peptide antibody was raised against double-stranded RNA-binding domain 4. B, quantitative RT-PCR results of stau-1 mRNA expression during C. elegans development. mRNA levels were normalized according to total amount of RNA. C, Western blot using the STAU-1 peptide antibody on mixed stage lysate from wild-type, stau-1(ΔdsRBD2), and stau-1(ΔdsRBD4) animals. The stau-1 mutant lysates served as specificity controls. In vitro translated STAU-1 protein served as a positive control for the STAU-1 antibody. Tubulin served as a loading control. Numbers to the left indicate the approximate molecular mass in kDa according to the Fermentas PageRuler Plus Protein Ladder. D, Western blot using the STAU-1 peptide antibody on wild-type lysate from various developmental stages. In vitro translated STAU-1 protein served as a positive control for the STAU-1 antibody. Tubulin served as a loading control. Protein levels were normalized according to total amount of protein. Error bars, S.D.