FIGURE 5.

IP RT-PCR validation of RIP-Chip results. A, total RNA was isolated from immunoprecipitate inputs or pellets, reverse transcribed, and PCR-amplified using gene-specific primers. act-1 served as a loading control. M05B5.3 is a transcript that did not appear on our list of STAU-1-associated mRNAs. Negative control samples did not contain any PCR template and control for DNA contamination. B, list of major DAVID terms associated with STAU-1 targets. Count, refers to the number of STAU-1 targets within each group.