FIGURE 6.

stau-1 mutations enhance exogenous RNAi. Images represent the phenotypes of wild-type, stau-1(ΔdsRBD2), stau-1(ΔdsRBD4), and eri-1(mg366) animals after no RNAi (A–D), dpy-13(RNAi) (E–H), and lir-1(RNAi) (I–L). Both stau-1(ΔdsRBD2) and stau-1(ΔdsRBD4) exhibit enhanced RNAi phenotypes compared with wild-type. eri-1(mg366) was used as a positive control. Plus signs indicate the severity of the dpy-13(RNAi) phenotype: + indicates mildly dumpy, whereas +++++ indicates extremely dumpy. Percentages indicate the degree of lethality after lir-1(RNAi), and n signifies the number of total animals that were scored. Animals were considered “dead” if the pharynx was no longer pumping. Animals that no longer moved but still had a functioning pharynx were scored as “alive.”