TABLE 3.
Functional properties of mutant AT1 receptors
HEK293 cells expressing mutant AT1 receptors were assayed for their IP1 production as described under “Experimental Procedures.” EC50 values are expressed as the means ± S.D. of values obtained in n independent experiments performed in duplicate. ND, no detectable response.
| EC50 | n | |
|---|---|---|
| nm | ||
| AT1 | 2.4 ± 1.5 | 12 |
| N111G-AT1 | 3.3 ± 1.1 | 6 |
| N111W-AT1 | ND | 7 |
| N46G-AT1 | 35.8 ± 13.5 | 6 |
| N111G/N46G-AT1 | ND | 6 |
| D74N-AT1 | ND | 5 |
| N111G/D74N-AT1 | ND | 5 |
| F77A-AT1 | 2.1 ± 1.1 | 7 |
| N111W/F77A-AT1 | 3.7 ± 1.8 | 7 |