TABLE 1.
Specific activities of Ape1 and mutant derivatives
| Enzyme | Specific activitya |
|
|---|---|---|
| μmol·min−1·mg−1 | %b | |
| Ape1a wild-type | 10.4 | 100 |
| G78A | 0.473 ± 0.0653 | 4.54 |
| D79A | 0.0448 ± 0.00154 | 0.431 |
| S80A | 0.00626 ± 0.000247 | 0.0602 |
| H81F | 2.61 ± 0.0866 | 25.1 |
| N235A | 1.05 ± 0.1035 | 10.1 |
| G236A | 2.60 ± 0.0265 | 25.0 |
| T267A | 1.01 ± 0.0343 | 9.71 |
| N268L | 0.0240 ± 0.00626 | 0.231 |
| N268Q | 1.07 ± 0.0447 | 10.3 |
| N268S | 0.0866 ± 0.00619 | 0.832 |
| D366A | 0.209 ± 0.0109 | 2.01 |
| V368A | 0.965 ± 0.00738 | 9.28 |
| H369A | 0.00666 ± 0.000351 | 0.064 |
a Activity assayed in triplicate using 2 mm p-nitrophenyl acetate as substrate in 50 mm sodium phosphate buffer, pH 6.5, at 25 °C (n > 3, ± S.D).
b Values as percentage of wild-type Ape1a.