FIGURE 3.

Effect of NPAS4 overexpression on neurite outgrowth induced by DM in Neuro2a cells. A, representative photographs showing immunocytochemical analysis of Npas4- or mock vector-transfected cells (Myc-positive). Scale bar, 20 μm. B, quantitative analysis of neurite length. Values indicate the mean ± S.E. (left) and the cumulative frequency of neurite length (right). (n = 88 for Npas4 vector transfection (Npas4), n = 42 for mock vector transfection (Mock), and n = 160 for nontransfection (Control).) *, p < 0.01 versus mock and control (one-way ANOVA followed by Dunnett's test, confirmed by the Kolmogorov-Smirnov test).