FIGURE 5.
Modulation of Na,K-ATPase α3 activity by activation of the PKA pathway. Hippocampal neurons were superfused with a solution containing cAMP analogs, inhibitors of cAMP degrading enzymes, and phosphatase inhibitors. A, box plot of the Ip ratio (proportion of second K+-evoked Ip to first K+-evoked Ip) during control and PKA-stimulating conditions. The Ip was measured as the average net current recorded during 8 mm extracellular K+. Control, n = 6 neurons; PKA activation, n = 9 neurons. B, box plot of Na+ clearance rate in the presence of vehicle (dimethyl sulfoxide, 0.11%) or drugs activating the PKA pathway. Dimethyl sulfoxide, n = 19 dendrites from four experiments; PKA, n = 15 dendrites from four experiments. *, p < 0.05, using one-way ANOVA with Bonferroni correction.