FIGURE 2.

Radio silica thin layer chromatogram of reaction products produced by PpORS from fatty acyl-CoA starter substrates of varying chain lengths and [2-¹⁴C]malonyl-CoA. The number below each lane indicates the chain length of the fatty acyl-CoA substrate; e.g. 6 for C₆-CoA and 8 for C₈-CoA, etc. The control sample (lane c) received [2-¹⁴C]malonyl-CoA only. Specific activity of PpORS when determined with C₂₄-CoA was 0.32 ± 0.12 picokatals/mg (±S.D., n = 3). PpORS also produced additional minor products from C₈- to C₁₆-CoA substrates. The yields of these minor products were 3–7% of those of the major products (3j–3e) and remained uncharacterized.