FIGURE 3.

Effect of rapamycin, Torin1, and FK506 on PMT-induced rpS6 and S6K1 phosphorylation. A, serum-deprived Swiss 3T3 cells were left untreated or treated with 50 ng/ml of PMT for 24 h followed by treatment with rapamycin (30 nm) (upper panel), Torin1 (150 nm) (middle panel), or FK 506 (200 nm) (lower panel) for 90 min. Protein extracts were subjected to immunoblotting with antibodies against rpS6 and pS6 Ser-235/236 and pS6 Ser-240/244. B, serum-starved cells were treated with PMT for 6 h followed by rapamycin treatment as above and equal amounts of protein were separated by SDS-PAGE. Immunoblots were developed using S6K1 and pS6K1 Thr-389 antibodies as indicated.