FIGURE 6.

C-PAF treatment of normal canine right atrial tissue increases the phosphorylation of TASK-1 at Thr-383. Normal canine right atrium was incubated with C-PAF (0–2 μm) for 15 min, after which crude membrane fractions of the homogenized tissue were prepared and analyzed by Western blotting for the presence of total TASK-1 and Thr(P)-383 TASK-1. A depicts a representative blot of Thr(P)-383 (top panel) and total TASK-1 (bottom panel) isolated from C-PAF-treated atrial myocardium. B depicts the summary of data from six experiments. C-PAF treatment resulted in a significant increase in Thr(P)-383 relative to total TASK-1 (*, p < 0.05, 2 μm versus 0 μm; repeated measures analysis of variance). C depicts the summary of data from six independent experiments that included the addition of the PKC inhibitor BIM-1 (5 μm). Inhibition of PKC significantly reduced the C-PAF-dependent phosphorylation of Thr-383 (**, p < 0.01, 2 μm versus 0 μm or versus 2 μm + BIM-1; repeated measures analysis of variance). IB, immunoblot.