Fibril formation was monitored by ThT fluorescence for apoA-I variants incubated at pH 7.4.
A, ▵, apoA-I WT; ▾, G26R; □, 1–83; ●, 1–83/G26R. Protein concentration was 0.3 mg/ml. a. u., arbitrary units. Error bars indicate S.E. B, effect of protein concentration on ThT fluorescence of apoA-I 1–83/G26R. ▴, 0.1 mg/ml; ●, 0.3 mg/ml; ▿, 0.5 mg/ml. Error bars indicate S.E.