FIGURE 3.

Morphology of S. cerevisiae W303 hsp104Δ cells carrying variants of the HSP104 gene. A, the upper panel shows representative microscopic images of cells. For toxic K358E and D484K mutants, besides an apparent increase of size, numerous lysed cells were also observed. Lower panel, histograms of cell diameters for respective HSP104 mutants (∼100 cells were measured) are shown. B, fluorescence microphotographs of S. cerevisiae cells stained with TRITC-phalloidin to visualize actin structures are shown.