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Temporal regulation of inflammatory cell gene expression by CL 316,243. Mice were injected with 0.5 mg/kg CL 316,243 or PBS intraperitoneally for 30 min to 24 h as indicated. Visceral adipose tissue was isolated, RNA was extracted and quantitative RT-PCR was performed for A, Itgam (CD11b), B, CD68, C, Itgax (CD11c), D, EMR1 (F4/80), E, Il1β, F, CCL2 (MCP-1), G, TNFα, and H, IL-6. Data represent average gene expression as compared with 36b4 ± S.E. (n = 3–7, *; p < 0.05).
