FIGURE 7.

Interaction of RapA2 with bacterial soluble EPS. A, binding of RapA2 to different soluble polysaccharides was examined. The protein was added to the EPS preparation, and after sedimentation of the EPS by centrifugation at 100,000 × g, the pellet (even numbers) and supernatant fractions (odd numbers) were assayed for the presence of proteins by SDS-PAGE. B, shown is inhibition of the binding of RapA2 to EPS of R. leguminosarum bv. viciae strain 3841 by different soluble EPS preparations. Inhibitory EPS was serially diluted by a factor of 10, starting with a concentration of 100 μg ml⁻¹. R.l.v., R. leguminosarum bv. viciae; R.l.t., R. leguminosarum bv. trifolii; PK, pretreatment with proteinase K.