Table 1. Transduction titers of VA-deleted AdVs.
| AdV | Type | Transduction titer copies/mL (×107) | Ratio (%) |
|---|---|---|---|
| AxdV-4FVF-GFP | pre-vector | 37 | 100 |
| AxdV-4F-GFP | VA-deleted | 5 | 14 |
| AxdV-FVF-GFP | pre-vector | 60 | 100 |
| AxdV-F-GFP | VA-deleted | 7 | 12 |
| AxdV-4FVF-NCre | pre-vector | 47 | 100 |
| AxdV-4F-NCre | VA-deleted | 4 | 8 |
| AxdV-FVF-SRChe | pre-vector | 20 | 100 |
| AxdV-F-SRChe | VA-deleted | 2 | 12 |
The transduction titer of the control FG AdV, AxEFGFP, was 83 × 107 copies/mL. The transduction titer was called the relative vector titer (rVT)12, which is about five-times lower than the conventional TCID50 titer of the pre-virus measured using 293 cells (see Methods).