Table 2. Expression levels of VAI and VAII RNAs measured using qPCR.
| VA RNAs | |||||
|---|---|---|---|---|---|
| I | II | ||||
| AdVs | Type | Copies ×108 | Ratio (%) | Copies ×108 | Ratio (%) |
| AxdV-4FVF-GFP | pre-vector | 4.47 ± 0.81 | 100 | 1.36 ± 0.15 | 100 |
| AxdV-4F-GFP | VA-deleted | 0.02 ± 0.00 | <1 | ND | <1 |
| AxdV-FVF–GFP | pre-vector | 1.81 ± 0.06 | 100 | 1.83 ± 0.32 | 100 |
| AxdV-F-GFP | VA-deleted | 0.05 ± 0.00 | 2.9 | 0.03 ± 0.00 | 1.4 |
| AxCAGFP | control FG AdV | 5.97 ± 0.83 | 4.17 ± 0.16 | ||
HuH-7 cells were infected with 50 μL of conventional stock of the pre-vector and the second stock of VA-deleted AdV. The method for preparation of the second stock is described in the Method section.