FIG. 3.

Effect of cytostatic drugs on AAV transduction. Transduction frequency in HeLa (a), HT-1080 (b), and U-2 OS (c) cells. After treatment with cytostatic drugs, 50,000 cells were transduced with AAV.donor-REx at 20 gc/cell (HeLa, U-2 OS) or 50 gc/cell (HT-1080) and assessed by flow cytometry four days later. Graphs represent average percentage of REx-positive cells with standard deviation, and asterisks mark significant (p<0.05) differences in transduction frequency of treated vs. nontreated cells. Control infections were performed with AAV.donor-SceI. E, etoposide; H, hydroxyurea; I, indirubin-3′-monoxime; M, L-mimosine; V, vinblastine; w/o, without cytostatics.