FIG. 4.

Targeting ratio. I-SceI-induced AAV-mediated gene targeting was performed as described in Figure 1. Quantitative PCR was performed on genomic DNA extracted 30 days post transduction with primer pairs (see Fig. 1a) to quantify the number of randomly integrated AAV vector genomes (RI) or target locus (TL) as an internal control. The targeting ratio is indicated as the quotient between the number of gene-targeting events (percent EGFP-positive cells at day six) and RI events (I-SceI copies per TL at day 30). Graph represents average targeting ratio with standard deviation.