Table 3.
Relative Gene Expression in Renal Tissues Before and After Experimental Rhabdomyolysis
| SOD-1 | SOD-2 | GPx 1 | NFκB | TNFα | ATR I | ATR II | |
|---|---|---|---|---|---|---|---|
| Sham | 1.0 (0.1) | 1.0 (0.0) | 1.0 (0.0) | 1.0 (0.1) | 1.0 (0.0) | 1.0 (0.0) | 1.0 (0.0) |
| RM | 2.1 (0.1)* | 7.1 (1.1)* | 2.4 (2.2)* | 1.0 (0.2) | 15.7 (4.4)* | 13.1 (4.1)* | 13.3 (5.5)* |
| Se+ | 2.7 (0.4)* | 4.7 (1.1)*# | 3.7 (2.5)* | 1.9 (0.2) | 17.9 (5.8)* | 7.0 (1.7)*# | 13.4 (5.6)* |
| Se++ | 1.3 (0.4)** | 2.1 (0.4)**# | 2.3 (0.9)* | 0.8 (0.4) | 8.4 (3.2) | 7.0 (1.6)*# | 6.9 (2.3)* |
Gene regulation studies were performed as described in the Methods section. Data are expressed as mean±(SD); n=6 for sham, RM, Se+, and Se++ groups. Antioxidant stress elements (SOD-1/2 and GPx-1), inflammation markers (TNF and NFκB), and receptors for angiotensin (ATRI and ATRII) were measured and normalized against the corresponding β-actin housekeeping gene. Each treatment group was then expressed as a fold-change compared to the sham (arbitrarily assigned unitary value).
*
Different to the sham; p<0.01.
#
Different to the RM group; p<0.05.
**
Different to the Se+ group; p<0.05.