Table 4.

Primer Sequences for Q-PCR Analysis

Gene	Forward primer	Reverse primer	Annealing temperature (°C)
ATRI	5′-ATGCCAGTGTGTTTCTGCTC-3′	5′-CCAATGGGGAGTGTTGAGTT-3′	60°C
ATRII	5′-GTGTCCAGCATTTACATCTTCA-3′	5′-CACCAAACAAGGGGAACTAC-3′	60°C
β-ACTIN	5′-AGCCATGTACGTAGCCATCC-3′	5′-CTCTCAGCTGTGGTGGTGAA-3′	60°C
GPx-1	5′-TGAGAAGTGCGAGGTGAATG-3′	5′-AACACCGTCTGGACCTACCA-3′	60°C
NF-κB1	5′-TTCCCCACACTGTAAACCAA-3′	5′-AGCAAGTGTAATCCAATAGC-3′	60°C
SOD-1	5′-CCACTGCAGGACCTCATTTT-3′	5′-TCTTCATTTCCACCTTTGCC-3′	60°C
SOD-2	5′-GGCCAAGGGAGATGTTACAA-3′	5′-GCTTGATAGCCTCCAGCAAC-3′	60°C
TNFα	5′-AGGGTACCACAGAAAGATGC-3′	5′-GGAGATGAGACCCTTAGGTT-3′	58°C

Oligomers were synthesized by Sigma Aldrich (Sydney, Australia) and β-actin was employed as a housekeeping gene in all reverse transcriptase-PCR studies. The corresponding annealing temperatures employed in the RT-PCR reactions are also listed. ATRI/II, angiotensin receptors I and II; GPx-1, glutathione peroxidase-1; NFκB, nuclear factor kappa B; SOD-1/2, superoxide dismutase 1 and 2; TNF, tumor necrosis factor.