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. Author manuscript; available in PMC: 2013 May 22.
Published in final edited form as: ACS Nano. 2012 Apr 24;6(5):3954–3966. doi: 10.1021/nn300149c

Figure 1.

Figure 1

Precise size control of drug/dye-silica nanoconjugates (NCs). (a) Preparation of pyrene-silica nanoconjugates (Pyr-NCs) with discrete size ranging from 20 to 200 nm. Three to five separate batches of Pyr-NCs for each size were prepared to demonstrate the consistency in size control and batch-to-batch reproducibility. The diameters of NCs were determined by measuring the particle size in a representative SEM image containing at least 100 particles (mean ± standard deviation). The difference of NC diameters between each size group is extremely statistical significant with 99.9% confidence (Student’s t-test (two-tailed), ***p < 0.001). The SEM images of NCs at each size (20, 35, 50, 65, 80, 100 and 200 nm) were displayed; the higher resolution images of NCs of the corresponding size were shown in the inset. (b) Preparation of different drug/dye-silica NCs with sizes of 50 nm and 20 nm. (c) The SEM images of Cpt20 and Cpt-N20 as the examples to show the excellent size control and monodispersity of NCs with sizes of 20 nm.