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. 2013 Jan 3;9(1):94–107. doi: 10.7150/ijbs.5246

Figure 2.

Figure 2

AGE-LDL induced IL-6 and IL-8 expression in a time- and dose-dependent manner. (A-B) HK-2 cells were treated with native LDL (100μg/ml) or indicated amount of AGE-LDL for 12 h. The mRNA level of IL-6 and IL-8 was examined by real time PCR (A) and the protein level of IL-6 and IL-8 was measured by ELISA (B). (C-D) HK-2 cells were treated with native LDL (100μg/ml) or AGE-LDL (100μg/ml) for indicated time period. The mRNA level of IL-6 and IL-8 was examined by real time PCR (C) and the protein level of IL-6 and IL-8 was measured by ELISA (D). (E-F) AGE-LDL-induced IL-6 production was not caused by LPS contamination. HK-2 cells were pretreated with PMX-B (10μg/ml) for 2 h before adding AGE-LDL or LPS. The mRNA level of IL-6 was examined by real time PCR (E) and the protein level of IL-6 was measured by ELISA (F). *P<0.05 versus untreated cells. #P>0.05 versus AGE-LDL treated cells. §P<0.05 versus LPS treated cells. Data are expressed as mean±SD of three independent experiments.