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Open lumen lymphatic ducts with disseminated cells within mucosa. CRC tissue sections from node-negative (A-B) and node-positive (C-D) cases were double immunostained for CK-19 (red) and D2-40 (brown). Circles identify a lower magnification review of B and D. Thick and thin arrows identify budding cells and lymphatic ducts, respectively. Red arrows identify lymphatic ducts with disseminated cells that are only seen in the node-positive case. A&C: 80X. B&D: 400X.
