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. 2013 Jan 17;3(1):71–83.

Figure 1.

Figure 1

Altered activation of downstream BCR signaling pathways in IgH.ETμ tumors. Flow cytometric measurement of phosphorylation of Akt (A) and Erk (B) upon BCR stimulation in IgH.ETμ CLL cells versus wild-type (WT) B cells. MACS-purified CD19+ cells were stimulated for 5 minutes with 20 μg/mL goat anti-mouse Igκ and subsequently stained for phosphorylated Akt or Erk. C: Protein levels of c-Rel determined by western blotting in nuclear lysates of unstimulated (-) and αIgM stimulated (+) CD19+ MACS-sorted CLL cells and wild-type (WT) splenic B cells. D: Left: Btk expression levels in gated CD19+ wild-type (WT) splenic cells versus IgH.ETμ CLL cells were evaluated using flow cytometry. Background staining levels were determined in Btk-/- cells. Right: flow cytometric determination of median Btk expression levels in wild-type (filled circles) and IgH.ETμ CLL cells (open circles). MFI: median fluorescence intensity. For all analyses at least 4 CLL samples and 3 wild-type splenic B cell fractions were included; representative results are shown.