Table 3.
Effect of various favorable alleles for different functional polymorphisms of LcyE in five mono-genic segregating populations
| Polymorphism | Population | Pedigree | Genotype class | No. of kernels | LUT | ZEA | BCX | BC | ProA | Ratio |
|---|---|---|---|---|---|---|---|---|---|---|
| LcyE-5′TE | L1-P1 | CML486 | “2” (U) | 25 | 11.4 ± 0.6 | 21.7 ± 1.9 | 9.1 ± 0.3 | 0.76 ± 0.04 | 5.04 ± 0.41 | 0.36 ± 0.01 |
| L1-P2 | Carotenoid Syn3-FS11-4-3-B–B–B | “4” (F) | 25 | 5.9 ± 0.4 | 14.3 ± 2.1 | 13.0 ± 1.2 | 1.10 ± 0.06 | 8.1 ± 0.70 | 0.21 ± 0.02 | |
| L1-F2 | CML486/Carotenoid Syn3-FS11-4-3-B–B–B | “2” | 57 | 4.5 ± 0.2 | 9.0 ± 0.8 | 6.1 ± 0.5 | 0.69 ± 0.02 | 7.51 ± 0.74 | 0.28 ± 0.00 | |
| “4” | 33 | 2.4 ± 0.3 | 9.01 ± 1.8 | 6.7 ± 0.8 | 0.78 ± 0.04 | 8.28 ± 1.17 | 0.14 ± 0.00 | |||
| “H” | 79 | 2.4 ± 0.4 | 8.01 ± 1.4 | 6.72 ± 1.1 | 0.68 ± 0.09 | 8.08 ± 0.50 | 0.16 ± 0.03 | |||
| LcyE-5′TE | L2-P1 | DRB-F2-60-1-1-1-BB/[BETASYN]BC1-9-#-B-B | “2” (U) | 25 | 0.7 ± 0.05 | 4.1 ± 0.3 | 3.8 ± 0.2 | 0.56 ± 0.04 | 2.22 ± 0.37 | 0.15 ± 0.01 |
| L2-P2 | CML297 | “4” (F) | 25 | 2.9 ± 0.03 | 10.4 ± 0.4 | 8.3 ± 0.4 | 0.57 ± 0.03 | 5.12 ± 0.51 | 0.09 ± 0.02 | |
| L2-F2 | CML-297-B/DRB-F2-60-1-1-1-BB/[BETASYN]BC1-9-#-B | “2” | 42 | 1.0 ± 0.05 | 3.7 ± 0.6 | 4.4 ± 0.5 | 0.59 ± 0.05 | 5.63 ± 0.34 | 0.12 ± 0.03 | |
| “4” | 46 | 0.7 ± 0.05 | 3.8 ± 0.4 | 3.3 ± 0.6 | 0.38 ± 0.02 | 4.13 ± 0.39 | 0.09 ± 0.01 | |||
| “H” | 85 | 1.3 ± 0.12 | 4.0 ± 0.8 | 4.2 ± 0.8 | 0.51 ± 0.06 | 5.30 ± 0.38 | 0.16 ± 0.01 | |||
| LcyE-5′TE | L3-P1 | MAS[206/312]-23-2-1-1-B–B–B/[BETASYN]BC1-11-3-1-#-B | “2” (U) | 25 | 1.5 ± 0.1 | 2.4 ± 0.4 | 2.5 ± 0.2 | 0.96 ± 0.05 | 2.4 ± 0.26 | 0.26 ± 0.01 |
| L3-P2 | CML297 | “4” (F) | 25 | 0.6 ± 0.05 | 4.6 ± 0.3 | 2.4 ± 0.1 | 0.47 ± 0.06 | 1.68 ± 0.16 | 0.09 ± 0.02 | |
| L3-F2 | MAS[206/312]-23-2-1-1-B-B-B/[BETASYN]BC1-11-3-1-#-B/CML297 | “2” | 29 | 1.1 ± 0.3 | 3.1 ± 0.2 | 2.6 ± 0.3 | 0.86 ± 0.1 | 2.18 ± 0.16 | 0.17 ± 0.02 | |
| “4” | 19 | 0.7 ± 0.06 | 3.0 ± 0.5 | 2.4 ± 0.4 | 0.96 ± 0.14 | 2.20 ± 0.07 | 0.11 ± 0.02 | |||
| “H” | 28 | 0.7 ± 0.05 | 2.3 ± 0.4 | 2.3 ± 0.4 | 0.91 ± 0.08 | 2.09 ± 0.24 | 0.12 ± 0.01 | |||
| LcyE-5′TE | L4-F2 | Hi27XCML328-F2 | “2” (U) | 54 | 0.9 ± 0.12 | 12.2 ± 1.6 | 4.2 ± 0.3 | 1.10 ± 0.2 | 3.21 ± 0.42 | 0.05 ± 0.01 |
| “1” (F) | 35 | 0.6 ± 0.08 | 11.8 ± 0.9 | 4.5 ± 0.2 | 1.16 ± 0.1 | 3.44 ± 0.53 | 0.03 ± 0.01 | |||
| “H” | 32 | 0.5 ± 0.05 | 10.4 ± 1.8 | 4.9 ± 0.7 | 1.12 ± 0.1 | 3.57 ± 0.50 | 0.03 ± 0.01 | |||
| LcyE-3′Indel | L5-P1 | P72c1xCML-297 × CL-02410-3-1-1-B–B | “2” (U) | 25 | 6.0 ± 0.5 | 10.2 ± 0.9 | 3.1 ± 0.1 | 0.39 ± 0.02 | 1.94 ± 0.00 | 0.44 ± 0.03 |
| L5-P2 | CML297 | “1” (F) | 25 | 0.6 ± 0.08 | 4.6 ± 0.3 | 2.4 ± 0.1 | 0.47 ± 0.05 | 1.68 ± 0.16 | 0.09 ± 0.02 | |
| L5-F2 | P72c1xCML-297 × CL-02410-3-1-1-B/CML-297-B–B | “2” | 23 | 3.8 ± 0.2 | 9.2 ± 1.2 | 3.1 ± 0.4 | 1.00 ± 0.08 | 2.59 ± 0.04 | 0.29 ± 0.01 | |
| “1” | 24 | 2.9 ± 0.4 | 10.1 ± 0.6 | 3.5 ± 0.2 | 1.03 ± 0.1 | 2.77 ± 0.05 | 0.20 ± 0.00 | |||
| “H” | 42 | 3.1 ± 0.3 | 10.3 ± 0.4 | 3.71 ± 0.5 | 1.10 ± 0.05 | 2.96 ± 0.37 | 0.21 ± 0.01 |
Concentrations are expressed as μg g−1 dry weight (DW). Parental lines (P1 and P2); segregating populations (F2)
LUT Lutein, ZEA zeaxanthin, BCX betacryptopxanthin, BC beta-carotene, ProA total provitamin A carotenoids, Ratio ratio of α- to β-branch carotenoids