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. 1974 Sep;14(3):457–468. doi: 10.1128/jvi.14.3.457-468.1974

Temperature-Sensitive Mutants of Adenovirus Type 12 Defective in Viral DNA Synthesis

Nada Ledinko 1
PMCID: PMC355538  PMID: 4854616

Abstract

Ten temperature-sensitive (ts) mutants of adenovirus type 12 which produce plaques at 31 but not at 38.5 C have been isolated after mutagenesis with nitrosoguanidine or nitrous acid. The mutants have been classified into six separate complementation groups. DNA-DNA hybridizations have shown that at 38.5 C the ts 401 and 406 mutants of groups B and E, respectively, synthesized less than 10% of the normal level of viral DNA. The two mutants were also defective in the production of late proteins at the nonpermissive temperature, as shown by fluorescent-antibody tests and analysis by sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Genetic recombination between the ts viruses 401 and 406 has been demonstrated; the recombination frequency for the wild-type virus production was 17.7%. Both mutants induced an increase in thymidine kinase activity at 38.5 C. Moreover, the two viral DNA-defective mutants shut off host DNA synthesis at the restrictive temperature. It is striking that at 38.5 C ts virus 401 transformed two to eight times more hamster cells than the wild-type virus, whereas ts virus 406 transformed at a frequency similar to the wild-type virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Casto B. C. Adenovirus transformation of hamster embryo cells. J Virol. 1968 Apr;2(4):376–383. doi: 10.1128/jvi.2.4.376-383.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Casto B. C. Enhancement of adenovirus transformation by treatment of hamster cells with ultraviolet irradiation, DNA base analogs, and dibenz(a,h)anthracene. Cancer Res. 1973 Feb;33(2):402–407. [PubMed] [Google Scholar]
  3. Duff R., Knight P., Rapp F. Variation in oncogenic and transforming potential of PARA (defective SV40)-adenovirus 7. Virology. 1972 Mar;47(3):849–853. doi: 10.1016/0042-6822(72)90579-x. [DOI] [PubMed] [Google Scholar]
  4. Ensinger M. J., Ginsberg H. S. Selection and preliminary characterization of temperature-sensitive mutants of type 5 adenovirus. J Virol. 1972 Sep;10(3):328–339. doi: 10.1128/jvi.10.3.328-339.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRIED M. ISOLATION OF TEMPERATURE-SENSITIVE MUTANTS OF POLYOMA VIRUS. Virology. 1965 Apr;25:669–671. doi: 10.1016/0042-6822(65)90098-x. [DOI] [PubMed] [Google Scholar]
  6. Ginsberg H. S., Williams J. F., Doerfler W. H., Shimojo H. Proposed nomenclature for mutants of adenoviruses. J Virol. 1973 Sep;12(3):663–664. doi: 10.1128/jvi.12.3.663-664.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green M. Effect of oncogenic DNA viruses on regulatory mechanisms of cells. Fed Proc. 1970 May-Jun;29(3):1265–1275. [PubMed] [Google Scholar]
  8. Ishibashi M. Temperature-sensitive conditional-lethal mutants of an avian adenovirus (CELO). I. Isolation and characterization. Virology. 1971 Jul;45(1):42–52. doi: 10.1016/0042-6822(71)90111-5. [DOI] [PubMed] [Google Scholar]
  9. Kimura G., Dulbecco R. A temperature-sensitive mutant of simian virus 40 affecting transforming ability. Virology. 1973 Apr;52(2):529–534. doi: 10.1016/0042-6822(73)90348-6. [DOI] [PubMed] [Google Scholar]
  10. Ledinko N., Evans M. Enhancement of adenovirus transformation of hamster cells by N-methyl-N'-nitro-N-nitrosoguanidine, caffeine, and hydroxylamine. Cancer Res. 1973 Nov;33(11):2936–2938. [PubMed] [Google Scholar]
  11. Ledinko N., Fong C. K. Kinetics of nucleic acid synthesis in human embryonic kidney cultures infected with adenovirus 2 or 12: inhibition of cellular deoxyribonucleic acid synthesis. J Virol. 1969 Aug;4(2):123–132. doi: 10.1128/jvi.4.2.123-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ledinko N. Nuclear acidic protein changes in adenovirus-infected human embryo kidney cultures. Virology. 1973 Jul;54(1):294–298. doi: 10.1016/0042-6822(73)90141-4. [DOI] [PubMed] [Google Scholar]
  13. Ledinko N. Nucleolar ribosomal precursor RNA and protein metabolism in human embryo kidney cultures infected with adenovirus 12. Virology. 1972 Jul;49(1):79–89. doi: 10.1016/s0042-6822(72)80008-4. [DOI] [PubMed] [Google Scholar]
  14. Ledinko N. Stimulation of DNA synthesis and thymidine kinase activity in human embryonic kidney cells infected by Adenovirus 2 or 12. Cancer Res. 1967 Aug;27(8):1459–1469. [PubMed] [Google Scholar]
  15. Lundholm U., Doerfler W. Temperature-sensitive mutants of human adenovirus type 12. Virology. 1971 Sep;45(3):827–829. doi: 10.1016/0042-6822(71)90206-6. [DOI] [PubMed] [Google Scholar]
  16. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
  17. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology. 1968 Sep;36(1):126–136. doi: 10.1016/0042-6822(68)90122-0. [DOI] [PubMed] [Google Scholar]
  18. Milo G. E., Jr, Schaller J. P., Yohn D. S. Hormonal modification of adenovirus transformation of hamster cells in vitro. Cancer Res. 1972 Nov;32(11):2338–2347. [PubMed] [Google Scholar]
  19. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pringle C. R., Howard D. K., Hay J. Termperature-sensitive mutants of pseudorabies virus with differential effects on viral and host DNA synthesis. Virology. 1973 Oct;55(2):495–505. doi: 10.1016/0042-6822(73)90191-8. [DOI] [PubMed] [Google Scholar]
  21. Russel W. C., Newman C., Williams J. F. Characterization of temperature-sensitive mutants of adenovirus type 5--serology. J Gen Virol. 1972 Dec;17(3):265–279. doi: 10.1099/0022-1317-17-3-265. [DOI] [PubMed] [Google Scholar]
  22. Seemayer N. H., Defendi V. Analysis of minimal functions of simian virus 40. II. Enhancement of oncogenic transformation in vitro by UV irradiation. J Virol. 1973 Dec;12(6):1265–1271. doi: 10.1128/jvi.12.6.1265-1271.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shiroki K., Irisawa J., Shimojo H. Isolation and a preliminary characterization of temperature-sensitive mutants of adenovirus 12. Virology. 1972 Jul;49(1):1–11. doi: 10.1016/s0042-6822(72)80002-3. [DOI] [PubMed] [Google Scholar]
  24. Subak-Sharpe J. H. The genetics of herpesvirus. Cancer Res. 1973 Jun;33(6):1385–1392. [PubMed] [Google Scholar]
  25. Suzuki E., Shimojo H. A temperature-sensitive mutant of adenovirus 31, defective in viral deoxyribonucleic acid replication. Virology. 1971 Feb;43(2):488–494. doi: 10.1016/0042-6822(71)90320-5. [DOI] [PubMed] [Google Scholar]
  26. Suzuki E., Shimojo H., Moritsugu Y. Isolation and a preliminary characterization of temperature-sensitive mutants of adenovirus 31. Virology. 1972 Aug;49(2):426–438. doi: 10.1016/0042-6822(72)90495-3. [DOI] [PubMed] [Google Scholar]
  27. Suzuki E., Shimojo H. Temperature-sensitive formation of the DNA replication complex in adenovirus 31-infected cells. J Virol. 1974 Feb;13(2):538–540. doi: 10.1128/jvi.13.2.538-540.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Takahashi M. Isolation of conditional lethal mutants (temperature sensitive and host-dependent mutants) of adenovirus type 5. Virology. 1972 Sep;49(3):815–817. doi: 10.1016/0042-6822(72)90540-5. [DOI] [PubMed] [Google Scholar]
  29. Tibbetts C., Johansson K., Philipson L. Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA. J Virol. 1973 Aug;12(2):218–225. doi: 10.1128/jvi.12.2.218-225.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wilkie N. M., Ustacelebi S., Williams J. F. Characterization of temperature-sensitive mutants of adenovirus type 5: nucleic acid synthesis. Virology. 1973 Feb;51(2):499–503. doi: 10.1016/0042-6822(73)90450-9. [DOI] [PubMed] [Google Scholar]
  31. Williams J. F., Gharpure M., Ustacelebi S., McDonald S. Isolation of temperature-sensitive mutants of adenovirus type 5. J Gen Virol. 1971 May;11(2):95–101. doi: 10.1099/0022-1317-11-2-95. [DOI] [PubMed] [Google Scholar]
  32. Williams J. F., Ustacelebi S. Complementation and recombination with temperature-sensitive mutants of adenovirus type 5. J Gen Virol. 1971 Nov;13(2):345–348. doi: 10.1099/0022-1317-13-2-345. [DOI] [PubMed] [Google Scholar]

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