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. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: Int J Biochem Cell Biol. 2012 Nov 16;45(2):401–407. doi: 10.1016/j.biocel.2012.11.005

Mouse pulmonary microvascular endothelial cells were incubated for 24 h without or with BSO 0.1 mM and then evaluated for GSH content (A) and by DPLA (B). A. Cells after BSO treatment were incubated with 0.1 mM mCB for 20 min and then were examined for specific fluorescence by confocal microscropy (ex 400 nm; em470 nm). The decrease in fluorescence reflects decreased GSH content of the cells; B: Cells after BSO treatment were treated with t-BOOH (50 μM) for 2 h, then probed with mouse monoclonal anti-Prdx6 and rabbit polyclonal anti-πGST antibodies for DPLA. The IgG control indicates the background fluorescence. There was no effect of GSH depletion with BSO on the interaction of the two proteins.

Mouse pulmonary microvascular endothelial cells were incubated for 24 h without or with BSO 0.1 mM and then evaluated for GSH content (A) and by DPLA (B). A. Cells after BSO treatment were incubated with 0.1 mM mCB for 20 min and then were examined for specific fluorescence by confocal microscropy (ex 400 nm; em470 nm). The decrease in fluorescence reflects decreased GSH content of the cells; B: Cells after BSO treatment were treated with t-BOOH (50 μM) for 2 h, then probed with mouse monoclonal anti-Prdx6 and rabbit polyclonal anti-πGST antibodies for DPLA. The IgG control indicates the background fluorescence. There was no effect of GSH depletion with BSO on the interaction of the two proteins.